Vitamin E analogs limit in vitro oxidant damage to bovine mammary endothelial cells
نویسندگان
چکیده
Diseases that occur during the transition period are exacerbated when cows unable to cope with an increased pro-oxidant load results in oxidative stress. Dairy cattle routinely supplemented vitamin E analog ?-tocopherol mitigate severity of Nonetheless, stress remains a disease predisposing condition for many dairy cattle. A better method optimizing antioxidant functions is needed. ?-Tocopherol only 1 8 analogs E, all which have varying properties other mammals, albeit shorter physiological half-life compared ?-tocopherol. primary bovine mammary endothelial cell oxidant challenge model was used determine certain analogs. The aim this study if analogs, namely ?-tocopherol or ?-tocotrienol, antioxidative cells and these may protect cellular viability function from damage. Physiological (10 ?M) supraphysiological (50 concentrations ?-tocotrienol had greater capacity reduce accumulated reactive oxygen species derived nitric oxide donating antagonist, ?-tocopherol, after 30 min 6 h treatment. Further, decreased cytotoxicity amount than at like concentrations, whereas reduced lipid peroxidation apoptosis more effectively Last, (5 10 significantly slowed induced loss barrier integrity over 48-h using electrical cell-substrate impedance sensing system. Concerningly, drastically 5 ?M despite no apparent effect on concentrations. ?-Tocotrienol, however, also show significant reductions doses (25 50 ?M). Our suggest has activities reduces damage due as These data set foundation further investigation into types whole animal models.
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 2021
ISSN: ['0022-0302', '1525-3198', '1529-9066']
DOI: https://doi.org/10.3168/jds.2020-19675